Ppt applications of surfaceenhanced raman scattering sers. Surface enhanced raman spectroscopy sers is currently enjoying exponential exposure in terms of publications and there are some highly innovative approaches now being investigated to harness the power of this phenomenon. Surface enhanced raman spectroscopy sers normally done in a silver or gold colloid or a substrate containing silver or gold. Jan 18, 2017 this article discusses a surface enhanced raman spectroscopy sers based methodology to identify low doses raman spectrometer. However, it is still a challenge to produce highly sensitive and reusable sers substrates with a minimum fluorescence background. This is evident by the number of sers publications in the last decade, from 580 in 2005 to almost 2000 in 2014. Summary of operating principles of surface enhanced raman spectroscopy sers instrumentation technique. Present and future of surfaceenhanced raman scattering. Recent results, discussed below, show that roughness is not a requirement, however. Surface enhanced raman spectroscopy sers is a powerful vibrational spectroscopy technique that allows for highly sensitive structural detection of low concentration analytes through the amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Catalyst structures, adsorbates, and reaction intermediates can be observed in low. Mar 29, 2016 in this work, a novel platform for surfaceenhanced raman spectroscopy sers based chemical sensors utilizing threedimensional microporous graphene foam gf decorated with silver nanoparticles.
Introduction raman spectroscopy was discovered by c. Basic principles and applications basic principles resonance raman scattering surface enhanced raman scattering sers instrumentation spectrometer excitation sources raman in catalysis in situ cells in situ raman of working catalysts c. Applications of surfaceenhanced raman scattering sers powerpoint ppt presentation. Used extensively for identification of chemical constituents in liquid. One method to amplify weak raman signals is to employ surfaceenhanced raman scattering sers. Pdf food quality control by surface enhanced raman scattering. Practical applications of surfaceenhanced raman scattering sers timothy o. Surface enhanced resonance raman scattering and serrs. Surfaceenhanced raman scattering, or sers, is a commonly used sensing technique in which inelastic light scattering figure 1 by molecules is greatly enhanced by factors up to 10 8 or even larger, enabling singlemolecule sm sers in some cases when the molecules are adsorbed onto corrugated metal surfaces such as silver or gold nanoparticles nps. Aptamers that specifically bind with target toxin molecules are designed, and then utilized as the recognition element in the assay. Sers is a type of vibrational spectroscopy, which utilizes the interaction of light and matter to elucidate details of the chemical structure of a molecule. The raman signal from molecules adsorbed on certain metal surfaces can be five to six orders of magnitude stronger than the. This low efficiency is overcome in surface enhanced raman spectroscopy sers by placing the chemicals of interest in the vicinity of a scatterer, typically a surface or collection of.
Present and future of surfaceenhanced raman scattering acs. Surfaceenhanced raman spectroscopy sers is an advanced raman technique that enhances the vibrational spectrum of molecules adsorbed on or in the vicinity of metal particles andor surfaces. Surfaceenhanced raman spectroscopy or surfaceenhanced raman scattering sers is a surfacesensitive technique that enhances raman scattering by. Surfaceenhanced raman scattering sers in microbiology. Although surface enhanced raman scattering sers spectroscopy has traditionally been used as an in vitro analytic tool, in the past few years the first reports of the feasibility of in vivo imaging of cancer with biocompatible sers probes have emerged. The discovery of surfaceenhanced raman scattering sers was perhaps the most crucial advancement in the field. Optical detection and spectroscopy of single molecules and single nanoparticles have been achieved at room temperature with the use of surfaceenhanced raman scattering. Surface enhanced raman spectroscopy sers jeanne bonner phys 275 november 26, 2007 outline what is sers. A specialized version of raman spectroscopy is surface enhanced raman spectroscopy sers. Herein, we constructed an aptamerbased sensor for the sensitive and highly specific detection of shigella sonnei via surface enhanced raman spectroscopy sers analysis. In sers, the analyte molecule is absorbed onto a metallic, nanoscale surface to. Raman in 1928 it is a spectroscopic technique used to observe vibration, rotational, and other lowfrequency modes in a system. In our lab, surface enhanced raman spectroscopy sers is used to realize the sensitive detection of those environmental toxins.
We divide sers research into two different directions with different targets. Development of surface enhanced raman scattering sers spectroscopy. Surfaceenhanced raman spectroscopy sers is a precise and noninvasive analytical technique that is widely used in chemical analysis, environmental protection, food processing, pharmaceutics, and diagnostic biology. The pill could be a suspected fake if sers peaks typical of alprazolam are not observed from the xanax tablet. Graphene provides the ideal prototype two dimensional 2d test material to investigate sers. Reproducibility in surfaceenhanced raman spectroscopy. Surface enhanced raman spectroscopy or surface enhanced raman scattering sers is a surface sensitive technique that enhances raman scattering by molecules adsorbed on rough metal surfaces or by nanostructures such as plasmonicmagnetic silica nanotubes. In this feature article, we discuss in detail developmental bottleneck issues in raman spectroscopy in its early stages and surface enhanced raman spectroscopy sers in the past four decades. Surface enhanced raman scattering sers is a physical phenomenon first discovered in 1974. Apr 19, 2009 after over 30 years of development, surface enhanced raman spectroscopy sers is now facing a very important stage in its history. Unique spectral signatures provide means of molecular identification.
Surfaceenhanced raman scattering for direct protein. During the past decades, researchers have made great efforts towards an ideal sers substrate, mainly including pioneering works on the preparation of uniform metal nanostructure arrays by various nanoassembly and. Yet, explaining the enhancement of a spectroscopic signal by fouteen orders of magnitude continues to. Represents one of very few books fully dedicated to the topic of surface enhanced raman spectroscopy sers gives a comprehensive summary of the underlying physical concepts around sers. Surfaceenhanced raman scattering sers ppt video online. What is surface enhanced raman scattering, or sers. Surfaceenhanced raman spectroscopy sers is a vibrational spectroscopy technique with sensitivity down to the single molecule level that provides fine molecular fingerprints, allowing for direct identification of target analytes. In the last decade, we have seen spurring growth in academic and industrial interest in raman spectroscopy and its modifications, in particular, surfaceenhanced raman spectroscopy sers. Abstractpointofcare poc device development is a growing field that aims to develop lowcost, rapid, sensitive invitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere from modern clinics to remote and low resource areas.
Surface enhanced raman spectroscopy with a laser pointer light source and miniature spectrometer1 matthew a. In this work, a portable bacteriagrasping surface enhanced raman scattering sers chip for identification of three species of uropathogens escherichia coli cft 073, pseudomonas aeruginosa pao1 and proteus mirabilis prm1 directly from culture matrix was reported. It was discovered approximately thirty years ago 2,3,4. Surfaceenhanced raman scattering sers is one of the strongest and most enigmatic e.
The discovery showed promise to overcome the traditionally low sensitivity problem in raman spectroscopy. Understanding the surfaceenhanced raman spectroscopy. Extensive theoretical and experimental research, together with continuous developmen. Almost 30 years after the first reports on surfaceenhanced raman signals, the phenomenon of surfaceenhanced raman scattering sers is now well established. Surfaceenhanced raman spectroscopy sers utilizes the following effect. Sers is a sensitive, selective, and versatile technique, and. Surfaceenhanced raman spectroscopy with a laser pointer. Surfaceenhanced raman scattering university of illinois. Because of its readiness, sensitivity, and minimum sample preparation requirements, sers is being considered as a powerful technique for food inspection. Highlysensitive surfaceenhanced raman spectroscopy sers. Principles of surfaceenhanced raman spectroscopy 1st edition. Because of such complications, surface enhanced resonance raman spectroscopy serrs has been developed. Review of experimentation and results obtained using s slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Surface enhanced raman spectroscopy, or surface enhanced raman scattering, often abbreviated sers, is a surface sensitive technique that results in the enhancement of raman scattering by molecules adsorbed on rough metal surfaces.
This presentation covers the development of the sers system. Surface enhanced raman spectroscopy sers sensing in. The explosive development of nanoscience and nanotechnology has assisted the rapid development of sers, especially during the last 5 years. Surface enhanced raman scattering sers exploits surface plasmons induced by the incident field in metallic nanostructures to significantly increase the raman intensity. By contrast, surfaceenhanced raman spectroscopy sers, utilizes surface plasmons to enhance raman scattering by several orders of magnitude, without requiring different equipment than. Nov 30, 2014 surface enhanced raman spectroscopy sers is an intense ongoing hot topic because it is an attractive tool for sensing or detecting molecules in trace amounts. Jun 12, 2012 surface enhanced raman spectroscopy sers is an attractive analytical technique, which enables singlemolecule sensitive detection and provides its special chemical fingerprints. Surfaceenhanced raman scattering sers raman signals are inherently weak, especially when using visible light excitation and so a low number of scattered photons are available for detection. Surface enhanced raman spectroscopy sers utilizes the following effect. Nanofabrication of surfaceenhanced raman spectroscopy sers substrates. Sers has since been exploited for bioanalysis because of its high sensitivity and multiplexing capabilities. Surfaceenhanced raman spectroscopy combined with stable isotope probing to monitor nitrogen assimilation at both bulk and singlecell level. Allows indirect probing of vibrational states in molecules.
Introduction to surface enhanced raman spectroscopy sers. Ppt surface enhanced raman spectroscopy sers powerpoint. Provides a detailed analysis of plasmons and plasmonics. Surface enhanced raman spectroscopy sers for in vitro. Schmidt1, anja boisen1 1the danish national research foundation and villum foundationscenter for intelligent drug delivery and sensing using microcontainers and nanomechanics idun, department of micro and. Development of surface enhanced raman scattering sers. Recently, surfaceenhanced raman spectroscopy sersbased techniques have shown great potential for rapid, sensitive and reliable analyte detection due to its simple pretreatment, high sensitivity and spectral recognition 1826. Some of these advantages such as increased sensitivity and selectivity and therefore the possibility of small sample sizes and detection of small concentrations are invaluable in the field of forensics. The sers is manifested as an enhancement by many orders of magnitude of the intensity of raman ra. May 25, 2015 the usual purpose is to enhance the sensitivity e. Ters or nano raman brings you the best of both worlds.
Probing single molecules and single nanoparticles by. Sers utilizes the unique optical properties of nanostructured metallic substrates to enhance the intensity of the native raman signal of an adsorbed analyte by several orders of magnitude. Platelets obtained from patient specimens have been investigated by surfaceenhanced raman spectroscopy sers with the diodepumped solidstate laser. Fundamentals and applications of surfaceenhanced raman. Substrate for surfaceenhanced raman spectroscopy formed. Probing single molecules and single nanoparticles by surface. Pdf the surface enhanced raman scattering sers is a powerful method to. Raman scattering gives inherently weak signals, but the. Rapid and ultrasensitive surfaceenhanced raman spectroscopy. This phenomenon occurs when the molecules of interest are in very close proximity to the metal substrate, and is generally used for evaporated solutions or particlesurface characterization.
If so, share your ppt presentation slides online with. Surfaceenhanced raman spectroscopy applied to food safety. Frontiers a surfaceenhanced raman spectral library of. Surfaceenhanced raman spectroscopy or surfaceenhanced raman scattering sers is a surfacesensitive technique that enhances raman scattering by molecules adsorbed on rough metal surfaces or by nanostructures such as plasmonicmagnetic silica nanotubes. Since the discovery of the sers effect over 40 years ago, the technique has developed into an information rich and sensitive technique for the detection of trace analytes and biomolecules. Surfaceenhanced raman spectroscopicbased aptasensor for.
The articles collected in this themed issue are focused on the technique of surface enhanced raman scattering sers. Surface enhanced raman scattering, or sers, is a commonly used sensing technique in which inelastic light scattering figure 1 by molecules is greatly enhanced by factors up to 10 8 or even larger, enabling singlemolecule sm sers in some cases when the molecules are adsorbed onto corrugated metal surfaces such as silver or gold nanoparticles nps. Surface enhanced raman spectroscopy sers 1 surface enhanced raman spectroscopy sers bmen 489689. Princeton instruments surfaceenhanced raman spectroscopy. Tip enhanced raman spectroscopy ters tip enhanced raman ters or nanoraman. Analytical, biophysical and life science applications provides a wellbalanced account of the basic principles of sers, advances in the sers method, and applications of sers technology. With the advanced development of miniaturized raman spectroscopy, surface enhanced raman spectroscopy sers has extended its applications into the field of pointofcare testing poct and demonstrated its great significance by virtue of its noninvasive property and capability of fingerprint identification. Surfaceenhanced resonance raman scattering and serrs.
The enhancement factor can be as much as 10 10 to 10 11, which means the technique may detect single molecules. A key issue in sers detection is how to construct high active sers substrate. Understanding surface enhanced raman spectroscopy sers. In particular, most raman peaks have a narrow width suitable for multiplex anal. The phenomenon of sers was described as early as 1974, 49,50 and is understood to arise from a localized increase in the coupling between the. Individual silver colloidal nanoparticles were screened from a large heterogeneous population for special sizedependent properties and were then used to amplify the spectroscopic signatures of adsorbed molecules.
Surfaceenhanced raman spectroscopy for in vivo biosensing. Even 35 years after the discovery of surfaceenhanced raman spectroscopy sers much remains to be learned about the phenomenon. The initial steps toward a miniature, field portable sensor based on surface enhanced raman spectroscopy sers are presented. Moreover, since inexpensive and easily prepared nps are used as the raman signal enhancing media for sample molecules of low. Surfaceenhanced raman spectroscopy for antiplatelet. Sers is a specific raman spectroscopic technique that provides enhanced raman signals several orders of magnitude greater than normal for numerous raman active analyte molecules adsorbed onto rough metal surfaces. Surface enhanced raman spectroscopy has many advantages over its parent technique of raman spectroscopy. Optical detection and spectroscopy of single molecules and single nanoparticles have been achieved at room temperature with the use of surface enhanced raman scattering. Surfaceenhanced raman scattering sers has become a mature spectroscopic technique with the fast development in the past four decades, and its detection applications in the fields of chemistry, materials science, biochemistry and life sciences are rapidly expanding.
Surface enhanced raman spectroscopy sers raman spectroscopy is a versatile, noninvasive spectroscopic method for characterizing different materials with light. Surface enhanced raman spectroscopy and its application to. In sers, the analyte molecule is absorbed onto a metallic, nanoscale surface to achieve enhanced raman signals. Apr 10, 2018 surface enhanced raman spectroscopy sers is a powerful technique that is used to enhance sensitivity when using raman spectroscopy.
Surfaceenhanced raman scattering exhibits virtually all the advantages of conventional raman but adds quantitative ultrasensitivity up to single molecule detection, 90 92 to enhance its robust discriminatory ability. Ppt surfaceenhanced raman scattering sers powerpoint. Surface and tipenhanced raman spectroscopy sers and ters techniques exhibit highly localized chemical sensitivity, making them ideal for studying chemical reactions, including processes at catalytic surfaces. Despite its high specificity and sensitivity, the sers technique has not been established as a routine analytic method most likely due to the low reproducibility of the sers signal. Surface enhanced raman spectroscopy and its application to molecular and cellular analysis yun suk huh. It is based on measuring raman scattering, which is unique to all materials and allows the chemical composition of the sample to be analyzed. Surface enhanced raman spectroscopy sers was investigated as a method for the rapid qualitative analysis of synthetic piperazines. Sers is a phenomenon in which the raman intensity of a molecule is enhanced enormously up to 10 14fold when placed near a noble metal surface with high curvature, such as a silver or gold nanoparticle. A free powerpoint ppt presentation displayed as a flash slide show on id. The raman signal from molecules adsorbed on certain metal surfaces can be five to six orders of magnitude stronger than the raman signal from the same molecules in bulk volume. Coreshell nanostructureenhanced raman spectroscopy for.
A problem with sers spectra is that they can be difficult to interpret since the normal raman spectrum of a molecule is not necessarily the same as that of the same molecule adsorbed on a sers substrate. Portable bacteriacapturing chip for direct surfaceenhanced. Scientific products raman spectroscopy raman academy raman faqs what is surface enhanced raman scattering, or sers. Surfaceenhanced raman scattering sers consists in using the large local field enhancements that can exist at metallic surfaces under the right conditions, typically by profiting from localized surface plasmon resonances to boost the raman scattering signal of molecules at or close to the surface. Raman spectroscopy is commonly used in chemistry to provide a fingerprint by which molecules can be identified. Surface enhanced raman scattering sers is a technique which offers orders of magnitude increases in raman intensity, overcoming the traditional drawback of raman scattering its inherent weakness.
Surfaceenhanced raman scattering sers powerpoint ppt presentation. A composite material integrated of the raman active 4mba ligand of the eucomplex and citratestabilized au nanoparticles citau nps was synthesized and served as both active substrate and raman reporter. Surfaceenhanced raman spectroscopy sers inherits the rich chem. Surfaceenhanced raman spectroscopy sers utilizes specialized metal substrates to allow raman signal enhancement up to 10 orders of magnitude. During the past decade, the ability of surfaceenhanced raman spectroscopy sers to measure extremely low concentrations, such as mgl and below, and the availability of handheld raman spectrometers, has led to a significant growth in the number and variety of applications of sers to realworld problems. Surfaceenhanced raman spectroscopy sers can provide rich structural information with ultrahigh surface sensitivity, even down to the singlemolecule level, which makes it a promising tool for the in situ study of catalysis. The enhancement factor can be as much as 10 1410 15, which allows the technique to be sensitive enough to detect single molecules. Surface enhanced raman spectroscopy on a flat graphene. In the sers based analysis andor sensing system, the preparation of a lowcost, high. Most of these applications involve the measurement of drugs, such as quantifying. Surfaceenhanced raman spectroscopy for antiplatelet therapy. As a result, a set of optimal experimental parameters for platelets discrimination have been determined.